Analysis and design on low-power multi-Gb/s serial links

High speed serial links are critical components for addressing the growing demand for I/O bandwidth in next-generation computing applications, such as many-core systems, backplane and optical data communications. Due to continued process scaling and circuit innovations, today's CMOS serial link transceivers can achieve tens of Gb/s per pin. However, most of their reported power efficiency improves much slower than the rise of data rate. Therefore, aggregate I/O power is increasing and will exceed the power budget if the trend for more off-chip bandwidth is sustained. In this work, a system level statistical analysis of serial links is first described, and compares the link performance of Non-Return-to-Zero (2-PAM) with higher-order modulation (duobinary) signaling schemes. This method enables fast and accurate BER distribution simulation of serial link transceivers that include channel and circuit imperfections, such as finite pulse rise/fall time, duty cycle variation, and both receiver and transmitter forwarded-clock jitter. Second, in order to address link power efficiency, two test chips have been implemented. The first one describes a quad-lane, 6.4-7.2 Gb/s serial link receiver prototype using a forwarded clock architecture. A novel phase deskew scheme using injection-locked ring oscillators (ILRO) is proposed that achieves greater than one UI of phase shift for multiple clock phases, eliminating phase rotation and interpolation required in conventional architectures. Each receiver, optimized for power efficiency, consists of a low-power linear equalizer, four offset-cancelled quantizers for 1:4 demultiplexing, and an injection-locked ring oscillator coupled to a low-voltage swing, global clock distribution. Measurement results show a 6.4-7.2Gb/s data rate with BER < 10⁻¹² across 14 cm of PCB, and an 8Gb/s data rate through 4cm of PCB. Designed in a 1.2V, 90nm CMOS process, the ILRO achieves a wide tuning range from 1.6-2.6GHz. The total area of each receiver is 0.0174mm², resulting in a measured power efficiency of 0.6mW/Gb/s. Improving upon the first test chip, a second test chip for 8Gb/s forwarded clock serial link receivers exploits a low-power super-harmonic injection-locked ring oscillator for symmetric multi-phase local clock generation and deskewing. Further power reduction is achieved by designing most of the receiver circuits in the near-threshold region (0.6V supply), with the exception of only the global clock buffer, test buffers and synthesized digital test circuits at nominal 1V supply. At the architectural level, a 1:10 direct demultiplexing rate is chosen to achieve low supply operation by exploiting high-parallelism. Fabricated in 65nm CMOS technology, two receiver prototypes are integrated in this test chip, one without and the other with front-end boot-strapped S/Hs. Including the amortized power of global clock distribution, the proposed serial link receivers consume 1.3mW and 2mW respectively at 8Gb/s input data rate, achieving a power efficiency of 0.163mW/Gb/s and 0.25mW/Gb/s. Measurement results show both receivers achieve BER < 10⁻¹² across a 20-cm FR4 PCB channel

A Comparative Study of 20-Gb/s NRZ and Duobinary Signaling Using Statistical Analysis

A statistical analysis technique for estimating bit-error rate (BER) and eye opening is presented for both NRZ and duobinary signaling schemes. This method enables fast and accurate BER distribution simulation of a serial link transceiver including channel and circuit imperfections, such as finite pulse rise/fall time, duty cycle variation, and both receiver and transmitter forwarded-clock jitter. A comparison between 20-Gb/s NRZ and duobinary transmitters using this simulator shows that while duobinary transmission relaxes the requirements on the receiver equalizer due to the lower Nyquist frequency of the transmitted data, significant eye-opening and BER degradation can arise from clock non-idealities. The proposed statistical analysis is verified against traditional time-domain, transient eye-diagram simulations at 20-Gb/s, transmitted through measured s-parameter channel characteristics.Keywords: Bit-error rate (BER), eye diagram, jitter, duobinary, inter-symbol interference (ISI), serial link., non-return-to-zero (NRZ

α1A-Adrenergic Receptor Induces Activation of Extracellular Signal-Regulated Kinase 1/2 through Endocytic Pathway

G protein-coupled receptors (GPCRs) activate mitogen-activated protein kinases through a number of distinct pathways in cells. Increasing evidence has suggested that endosomal signaling has an important role in receptor signal transduction. Here we investigated the involvement of endocytosis in α1A-adrenergic receptor (α1A-AR)-induced activation of extracellular signal-regulated kinase 1/2 (ERK1/2). Agonist-mediated endocytic traffic of α1A-AR was assessed by real-time imaging of living, stably transfected human embryonic kidney 293A cells (HEK-293A). α1A-AR was internalized dynamically in cells with agonist stimulation, and actin filaments regulated the initial trafficking of α1A-AR. α1A-AR-induced activation of ERK1/2 but not p38 MAPK was sensitive to disruption of endocytosis, as demonstrated by 4°C chilling, dynamin mutation and treatment with cytochalasin D (actin depolymerizing agent). Activation of protein kinase C (PKC) and C-Raf by α1A-AR was not affected by 4°C chilling or cytochalasin D treatment. U73122 (a phospholipase C [PLC] inhibitor) and Ro 31–8220 (a PKC inhibitor) inhibited α1B-AR- but not α1A-AR-induced ERK1/2 activation. These data suggest that the endocytic pathway is involved in α1A-AR-induced ERK1/2 activation, which is independent of Gq/PLC/PKC signaling

Localization of Barley yellow dwarf virus Movement Protein Modulating Programmed Cell Death in Nicotiana benthamiana

Barley yellow dwarf virus (BYDV) belongs to Luteovirus and is limited only at phloem related tissues. An open reading frame (ORF) 4 of BYDV codes for the movement protein (MP) of BYDV gating plasmodesmata (PD) to facilitate virus movement. Like other Luteoviruses, ORF 4 of BYDV is embedded in the ORF3 but expressed from the different reading frame in leaky scanning manner. Although MP is a very important protein for systemic infection of BYDV, there was a little information. In this study, MP was characterized in terms of subcellular localization and programmed cell death (PCD). Gene of MP or its mutant (ΔMP) was expressed by Agroinfiltration method. MP was clearly localized at the nucleus and the PD, but ΔMP which was deleted distal N-terminus of MP showed no localization to PD exhibited the different target with original MP. In addition to PD localization, MP appeared associated with small granules in cytoplasm whereas ΔMP did not. MP associated with PD and small granules induced PCD, but ΔMP showed no association with PD and small granules did not exhibit PCD. Based on this study, the distal N-terminal region within MP is seemingly responsible for the localization of PD and the induction small granules and PCD induction. These results suggest that subcellular localization of BYDV MP may modulate the PCD in Nicotiana benthamiana

Scramblase TMEM16F terminates T cell receptor signaling to restrict T cell exhaustion

In chronic infection, T cells become hyporesponsive to antigenic stimulation to prevent immunopathology. Here, we show that TMEM16F is required to curb excessive T cell responses in chronic infection with virus. TMEM16F-deficient T cells are hyperactivated during the early phase of infection, exhibiting increased proliferation and cytokine production. Interestingly, this overactivation ultimately leads to severe T cell exhaustion and the inability of the host to control viral burden. Mechanistically, we identify TMEM16F as the dominant lipid scramblase in T lymphocytes that transports phospholipids across membranes. TMEM16F is located in late endosomes, where it facilitates the generation of multivesicular bodies for TCR degradation and signal termination. Consequently, TMEM16F deficiency results in sustained signaling and augmented T cell activation. Our results demonstrate that scramblase restricts TCR responses to avoid overactivation, ensuring a well-balanced immune response in chronic infectious disease

Regulatory Effect of DNA Topoisomerase I on T3SS Activity, Antibiotic Susceptibility and Quorum- Sensing-Independent Pyocyanin Synthesis in Pseudomonas aeruginosa

Topoisomerases are required for alleviating supercoiling of DNA during transcription and replication. Recent evidence suggests that supercoiling of bacterial DNA can affect bacterial pathogenicity. To understand the potential regulatory role of a topoisomerase I (TopA) in Pseudomonas aeruginosa, we investigated a previously isolated topA mutation using genetic approaches. We here report the effects of the altered topoisomerase in P. aeruginosa on type III secretion system, antibiotic susceptibility, biofilm initiation, and pyocyanin production. We found that topA was essential in P. aeruginosa, but a transposon mutant lacking the 13 amino acid residues at the C-terminal of the TopA and a mutant, named topA-RM, in which topA was split into three fragments were viable. The reduced T3SS expression in topA-RM seemed to be directly related to TopA functionality, but not to DNA supercoiling. The drastically increased pyocyanin production in the mutant was a result of up-regulation of the pyocyanin related genes, and the regulation was mediated through the transcriptional regulator PrtN, which is known to regulate bacteriocin. The well-established regulatory pathway, quorum sensing, was unexpectedly not involved in the increased pyocyanin synthesis. Our results demonstrated the unique roles of TopA in T3SS activity, antibiotic susceptibility, initial biofilm formation, and secondary metabolite production, and revealed previously unknown regulatory pathways

Artificial Synapse Consisted of TiSbTe/SiC_x:H Memristor with Ultra-high Uniformity for Neuromorphic Computing

To enable a-SiCx:H-based memristors to be integrated into brain-inspired chips, and to efficiently deal with the massive and diverse data, high switching uniformity of the a-SiC0.11:H memristor is urgently needed. In this study, we introduced a TiSbTe layer into an a-SiC0.11:H memristor, and successfully observed the ultra-high uniformity of the TiSbTe/a-SiC0.11:H memristor device. Compared with the a-SiC0.11:H memristor, the cycle-to-cycle coefficient of variation in the high resistance state and the low resistance state of TiSbTe/a-SiC0.11:H memristors was reduced by 92.5% and 66.4%, respectively. Moreover, the device-to-device coefficient of variation in the high resistance state and the low resistance state of TiSbTe/a-SiC0.11:H memristors decreased by 93.6% and 86.3%, respectively. A high-resolution transmission electron microscope revealed that a permanent TiSbTe nanocrystalline conductive nanofilament was formed in the TiSbTe layer during the DC sweeping process. The localized electric field of the TiSbTe nanocrystalline was beneficial for confining the position of the conductive filaments in the a-SiC0.11:H film, which contributed to improving the uniformity of the device. The temperature-dependent I-V characteristic further confirmed that the bridge and rupture of the Si dangling bond nanopathway was responsible for the resistive switching of the TiSbTe/a-SiC0.11:H device. The ultra-high uniformity of the TiSbTe/a-SiC0.11:H device ensured the successful implementation of biosynaptic functions such as spike-duration-dependent plasticity, long-term potentiation, long-term depression, and spike-timing-dependent plasticity. Furthermore, visual learning capability could be simulated through changing the conductance of the TiSbTe/a-SiC0.11:H device. Our discovery of the ultra-high uniformity of TiSbTe/a-SiC0.11:H memristor devices provides an avenue for their integration into the next generation of AI chips

The ZAR1 resistosome is a calcium-permeable channel triggering plant immune signaling

Nucleotide-binding, leucine-rich repeat receptors (NLRs) are major immune receptors in plants and animals. Upon activation, the Arabidopsis NLR protein ZAR1 forms a pentameric resistosome in vitro and triggers immune responses and cell death in plants. In this study, we employed single-molecule imaging to show that the activated ZAR1 protein can form pentameric complexes in the plasma membrane, The ZAR1 resistosome displayed ion channel activity in Xenopus oocytes in a manner dependent on a conserved acidic residue Glu11 situated in the channel pore. Pre-assembled ZAR1 resistosome was readily incorporated into planar lipid-bilayers and displayed calcium-permeable cation-selective channel activity. Furthermore, we show that activation of ZAR1 in the plant cell led to Glu11 -dependent Ca2+ influx, perturbation of subcellular structures, production of reactive oxygen species, and cell death. The results thus support that the ZAR1 resisto-some acts as a calcium-permeable cation channel to trigger immunity and cell death